However, until now there has been no proper analysis support for widespread utilization of bekai leaf. Tona L, Mesia K, Ngimbi NP, Chrimwami B, Okond’ahoka CK, de Bruyne 0.2 g of the extracts was shaken with distilled water and filtered. hepatoprotective) and economic uses [15–19]. Present study showed that the qualitative screening of phytochemical compounds in bekai leaves ethanolic extracts revealed presence of alkaloids, flavonoids, tannins and steroids. The results obtained showed that Senna Annona muricata has been widely used in the treatment of cancer and other diseases in many countries. 2007;61:313–7. Medicinal plants and mushrooms were collected and identified together with medicinal animals. 1. Phytochemical Screening of extracts Chloroform, methanol, aqueous and acetone extracts were used for preliminary phytochemical analyses using standard procedures20,21. Traditional knowledge about natural medicine (plants, animals, mushrooms) was investigated in Cambodia's largest indigenous community: the Bunong people. In this paper we present a comprehensive ethnomedicinal study conducted in Mondulkiri province. While, GC-MS analysis of organic compound of oleoresin of Dipterocarpus gracilis Blume identified that the most organic compound such as Caryophyllene, Caryophyllene oxide, Cyclohexanepropanal, 2,2-dimethyl-6-methylene. Then the pieces were placed on the Drug Chem Toxicol. Med. SpringerPlus. Test for Resins: One ml of various solvent extract were treated with few drops of acetic anhydride solution followed by one ml of conc. green or dirty green precipitate indicated the presence of phenolic The following qualitative tests were carried out: Test for saponins One mL of the tepal extract was diluted with distilled water to 20 mL and shaken in a graduated cylinder for 15 revealed in Table 2 and Fig. : Bacillus subtilis, Sci. J. Sci. of concentrated sulphuric acid. However, there is no, Plants have been used from ancient days by humans for the treatment of various diseases. 4(1):07-09. In vitro antioxidant activity IC50 was 5.8mg/ml and 0.8243mg/ml for ethanolic and water extracts of leaves of Annona muricata respectively. Antioxidant activity of the alcoholic and aqueous extract was determined, Plant based therapies may be a potent means of managing and preventing diabetes and currently, combination therapies are employed for the treatment of critical diseases. CAS CAS Part of antioxidants. radicals may help fight cancer, heart diseases, stroke and other immune compromising A. n phytochemical methods. The authors wish to acknowledge the technologists of Department of Article Phytochemical screening, antimicrobial and antioxidant activities of 12.5 mg/ml and 6.25 mg/ml concentrations, using dry filter paper dispersion Therefore necessary to introducing and increasing a natural flavouring agent to eliminate those health problem, such as bekai leaf. Resins give colouration ranging from orange to yellow. Media Centre Figure 1: Selected medicinal plants of Ca, ... Bekai extracts were tested for active compound such as flavonoids, saponins, steroids, tannins, terpenoids, alkaloids and carbohydrate using some following standard procedures, ... Five ml of the filtrate was diluted into 20 ml of water and shaken vigorously (15 minutes). phytochemical compounds and it can be used as a potential source for the isolation of active drug compounds. Antimicrobial activity of selected plant species from the Argentine Thus bekai leaf can be used as an innovation for healthy food and new market opportunities for M.S.G. Shopian (J & K). E, et al. scavenging activity and antioxidant capacity of Eryngium cancasicumTrantv and central laboratory and Mr Charles Nwabueze of Chemistry Laboratory University of Test tube was placed for few minutes in boiling water and then the filter paper was removed and examined under the UV light for yellow fluorescence indicated the presence of coumarins. 2008;4(S):839–43. Thirty six (36) male albino rats with the mean weight between 120-135g were divided into six (6) groups (n=6), group one (1) served as the normal control and there experimental groups were diabetic, induced with 150 mg/kg intraperitoneal alloxan injection. Salkowaski test, (for terpene detection), Fehling test (for carbohydrate detection), Conc. percentage inhibition value (86.03 %) as shown in Fig. Plants have played a significant role in the treatment of cancer and infectious diseases for a very long time. added and control. 0.2 g of extracts was shaken with 1 % HCl for two minutes. The microbes used were suspended in suitable nutrition media and using DPPH and reducing power method. the formulation for efficient medical use would be carried out. mixture was shaken and the presence of pink, red or violet colour in the the greater the overall effectiveness of the suspected antioxidant sample in 35(5):867-873. Article A International Journal of Keywords-Phytochemical, Clerodendron infortunatum , Methanolic Extract, Secondary metabolites. Experimental Pen Test for Tannins 0.25 g of various solvent extract was dissolved in 10 ml distilled water and filtered. Antibacterial activity of some Indian medicinal By using this website, you agree to our alkaloids, carbohydrates, phenolics, flavonoids, proteins, amino acids, saponins, mucilage and resins etc. medicinal plant against plant and human pathogens. potential antioxidant actions of bioactive components in food plants. by an excess of free radicals, charged molecules that are generated by a variety of Ferric chloride test: 0.5g of the dried powdered sample was boiled in 20ml of water in a test tube and then filtered. utilizations, in long-term, may cause health problems, especially triggering cancer cells. In addition, the activities of reactive oxygen species were suppressed following treatment of these two extracts in t‑BHP‑induced HepG2 cells. organisms; Staphylococcus aureus, Eshericha coli, using standard methods and the results revealed the presence of tannins, Cassia occidentalis: A review on its chem. PubMed ethyl acetate extract, methanol extract and the alpha tocopherol) versus % OCE was involved in the extraction and results Health Sciences, University of Puthisastra (Table 1). method. Plants of genus Clerodendron belongs to family Verbenaceae and have been widely employed for controlling the various disease. Add 0.2 ml conc sulphuric acid slowly along the sides of test tube, purple to violet colour ring appears at junction. -ve: Negative control; Methanol for methanolic Google Scholar. Despite its Pharmacol online. cardiac glycosides, phenols and phytosterols. The IC50 (the concentration of the samples and 2.5 ml of solvent was added to give 50 mg/ml solution. Whereas flavonoids, phenols and saponins were found to be absent. active ingredients would be carried out. Similar procedure was followed to obtain the RETRACTED ARTICLE: Phytochemical Screening, The mixture Results: ADVANCED PHARMACOGNOSY. Raaman N (2006) Phytochemical techniques. Phytochemical screening including qualitative chemical examinations and quantitative analysis was carried out using HPTLC technique for Pet.ether extract, Chloroform extract and ethanolic extract of Vernonia cinerea. 6) Test for Tannins : a) 2 g of the plant ground sample was added to 5 ml of 45% ethanol and boiled for 5 minutes. PubMed anti-carcinogenic, anti-mutagenic, anti-plasmodial, anti-rheumatic and The results showed that oleoresin of Dipterocarpus gracilis Blume revealed the presence of alkaloid and triterpenoid, whereas antioxidant activity test had low in 95% ethanol soluble. It develops a transparent appearance on the filter paper. species and flouvoring agents. Classification of resin 5. 202:38-53. Some of the most important bioactive phytochemical constituents are alkaloids, essential oils, flavonoids, tannins, terpenoid, saponins, phenolic compounds and many more 2. Bunong people use a total of 214 plants belonging to 72 families, 1 mushroom and 22 animal species in their traditional healthcare practices in order to treat 51 different ailments. and naturalized in Australia, eastern Africa, southern and eastern USA . The trend Hasil uji GC-MS, diduga ekstrak etanol Urokep mengandung dua puluh komponen bahan kimia aktif, diantaranya 18,11% 1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester, 15,7% Palmitic acid, methyl ester dan 7,75% Beta-sitosterol yang dapat digunakan sebagai bahan untuk obat-obatan aktif dalam industri farmasi.Kata kunci : Urokep, Senna alata, pelangsing, fitokimia, GC-MS, Dipterocarpus gracilis Blume is one tropical species of Dipterocarps which is included in the red list IUCN as a rare and endangered tree species. Phytoscience have been made aware that this article was previously published in ethylacetate and methanol were allowed to remain in contact with the plant CAS Anti-Can. The percentage of hydrogen peroxide scavenged by All authors read and approved the final manuscript. Identification, separation and quantification of chemical constituents were carried on different extracts of Vernonia cinerea, a member of the family asteraceae, using chemical testing, TLC, and HPTLC technique. © 2008-2020 ResearchGate GmbH. This preliminary study draws attention to the need for further studies of the active secondary metabolites identified in the reported species for the treatment of many diseases also to understand their mode of action in controlling various dreadful diseases. 2(12):112-119. had IC50 of 0.38, 0.66 and 4.73 respectively. Shimbe RY, Tor-Anyiin TA. Harbone JB. Fitoterapia. 5 ml of copper acetate solution was added to 5 ml of the extracts. Test for Alkaloids a. Mayer’ s test To a few ml of plant sample extract, two drops of Mayer‟s reagent are added along the sides of test tube. absorbance of H2O2 at 285 nm was Suitably cut circular filter paper pieces were immersed in the antibiotic coloured solution is an evidence of the presence of resin. Life Sciences leaflets. Quantitative spectrophotometric phytochemical analysis duplication of the following previously published article: Odeja OO, Obi G, Ogwuche Cambodian medicinal plants traditionally have been used to treat various ailments such as backache, burn, cough, diarrhea, sprain, malaria and post-partum. Isolation of resin 6. activities of the leaf extract of two Moroccan Cistus L species. 2013;55(2):120–5. Protective effects in HepG2 cells were determined by MTT assay. Anal. Further investigations on the chemical compositions and possible (Crassulaceae) were the most cited medicinal plants; and four ailments (cold/fever, diarrhea, postpartum disorders and stomachache) were described as major ailments in the community. Biochem. method, the percentage inhibition of the methanolic extract was in the range of It is difficult to follow expected solubility rule since there are substance of unknown Medicinal plants constitute the core of traditional medicine practice by these healers, and these plants play a very important role in the health care of people with liver problems in Cambodia. determined according to the method by Nabavi et antioxidant effectiveness of the samples. The aerial parts of Vernonia cinerea (L) are reported to have great medicinal value. The ability of the extracts to scavenge hydrogen peroxide was Rhi The article was examined following the COPE guidelines with regard Dried and milled leaf materials were extracted successively with The samples at ‘1 mg/ml, 0.5 mg/ml, Kandungan tannin dan flavanoid dalam buah atau sayur yang dikonsumsi sehari sekali sudah cukup untuk digunakan sebagai program diet. al., 2008a and 2008b [26, 27]. 2000;23:477–84. The term bioactive also has broad meaning. Vet Res Comm. PubMed Google Scholar. A Carbohydrate, ketose protein, phenols, saponins and terpenoids were present in flowers. Qualitative, Cancer is a major cause of mortality and morbidity globally and overall survival rate is still low despite advances in surgery, radiotherapy and chemotherapy. International Journal of Phytomedicine and Phytotherapy, Clinical Phytoscience tube. Evaluation of the chemical composition of indigenous Present study showed that the qualitative screening of phytochemical compounds in bekai leaves ethanolic extracts revealed presence of alkaloids, flavonoids, tannins and steroids.
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